Method for the production of $g(v)-aminoalkylsphonic acids

ABSTRACT

The invention relates to a method for the production of Ω-aminoalkylsulphonic acids of general formula (I), where R1 and R2=optionally substituted alkyl groups with 1 20 C atoms and n=a whole number from 2 6, whereby an amine of formula (II), where R1 and R2 have the above meanings is reacted with an alkyl dihalide of formula (III), where n has the above meaning and X 1  and X 2 =chlorine or bromine, with addition of alkali hydroxide at a pH of 8 10. The pH is then adjusted to a value of 0 1, by addition of a hydrohalic acid and excess alkyl dihalide is separated off, before the reaction solution is adjusted to a pH of 6 7.5 with alkali liquor, alkali sulphite is added and the product (I) formed at elevated temperate.

[0001] The present invention concerns a new process for the preparation of ω-aminoalkane-sulphonic acids in aqueous solution.

STATE OF THE ART

[0002] Aminoalkane-sulphonic acids belong to the group of the sulphonamides of the general formula I

[0003] whereby R₁ and R₂ are alkyl groups and n a whole number from 1 to 5.

[0004] The preparation of a group in these compounds with n takes place, according to the prior art, according to the general reaction equation:

[0005] The 1,3-proponesultone used for this process hereby reacts as highly reactive agent also with weak nucleophiles.

[0006] However, in the high reactivity is also based a high physiological potential.

[0007] Therefore, the alkane sultones are also classified as potentially cancer-producing and, for this reason, the use is disadvantageous. However, in general, the yield of this reaction generally lies at 95 to 98 percent so that hitherto it represents the technically most important manner of preparation.

[0008] Examples for technically used alkane-sulphonic acids are: 2-morpholino-ethane-sulphonic acid and 4-(2-hydroxy-ethyl)-1-piperazine-propane-sulphonic acid. These compounds serve, inter alia, as biological buffer substances in cell cultures.

[0009] EP 0 752 420 A1 describes a process for the preparation of 3-pyridinium-propane sulphone betaine (PPS)with avoidance of 1,3-propane-sultone, whereby pyridine is reacted with 1,3-dihalopropane and sodium sulphite in the presence of water and an alkyl halide as solvent.

[0010] However, the process is only suitable for tertiary amine components which carry no hydrogen on the amine nitrogen. In the case of the use of secondary amines, there namely takes place a deprotonisation of the tertiary ammonium salt formed by still not reacted secondary amine since the the secondary amine represents a stronger base than a tertiary amine and the resulting quaternary nitrogen carries an easily split off hydrogen. Liberated tertiary amine can thereby again react with the dihalopropane and therewith form undesired quaternary products in the sense of the following equation:

[0011] Besides the desired intermediate compound V, this leads to the no longer further reacting ammonium halide salt IV and the undesired quaternary salt VI. This leads to considerable yield reductions and high proportions of by-products.

[0012] Furthermore, there is given a high burdening of the solutions obtained with alkali halides. However, in the case of the required high purity of the end products, for example as biobuffer, this is not acceptable so that, according to the known preparation process, an isolation and purification of the solid aminoalkane-sultone acids must take place.

[0013] Thus, hitherto no access is open to sulphoalkylated, for example sulphopropylated, secondary amine compounds with exclusion of alkanesulphones.

[0014] Therefore, the task exists to find a process with which also ω-aminoalkane-sulphonic acids can be prepared economically without alkanesultones and which preferably also permits a purification of the solutions obtained without isolation of the solid products.

[0015] This task is solved according to the features of the main claim and promoted by the features of the subsidiary claims.

[0016] Consequently, the invention concerns a process for the preparation of aminoalkane-sulphonic acids, preferably as aqueous solution, without use of alkanesultones. Furthermore, the purification of the solutions obtained and the steps necessary for the obtaining of the pure substances. It is also a further object of the new process to obtain high yields as in the case of the alkanesultone process with equal product quality. Therefore, possible side reactions must be suppressed, as well as a possibly complete reaction of the materials used being achieved.

[0017] Since it is not possible to bring secondary amines directly to reaction with dihaloalkanes and alkali metal sulphite free of by-products, it is necessary to use a new process for the preparation.

[0018] One hereby starts from secondary amines which are brought to reaction with dihaloalkanes is such a manner that the addition of alkaline lye takes place. This is necessary in order to suppress possible side reactions. One works in water-containing media.

[0019] In a second step, the addition of hydrohalic acid to the reaction solution is necessary in order to achieve an acid pH range favourable for the further steps of the preparation process.

[0020] For the formation of the sulphonic acid, alkali metal sulphite is added to the reaction solution in amounts equimolar to the amine.

[0021] The amounts contained of alkali metal halides, as well as the by-products resulting in traces, act disturbingly for the direct use of the products obtained, for example of the buffer substance. In order to obtain the product in the purity required for the biobuffer solution, after the reaction a purification of the aqueous reaction medium must be carried out. It is hereby to be taken into account that per 1 mol of the reaction product at least 2 mol of alkali metal halide result. On the basis of a small excess Of alkali metal sulphite in the reaction batch, the proportion of alkali metal salts in the product solution is, however, also additionally increased by this amount. A depletion of the alkali metal halides and by-products can now, for example, be carried out by means of ion exchange processes. However, because of the high content of alkali metal halides formed, this cannot be carried out economically. The nanofiltration process alone does not suffice for technical reasons to achieve the required product purity since in the nanofiltration all negative divalent charged particles and larger molecules, such as result as by-products are also held back and thus remain in the product solution.

[0022] The product purification described in the present invention now refers to the combination of nanofiltration and ion exchange process for the economic separation of of the product from the mono- and divalent alkali halides and by-products resulting during the reaction. The process of nanofiltration used in the scope of this invention and the ion exchange by means of ion exchange resins are known.

[0023] The nanofiltration hereby serves for the depletion of the monovalent alkyl halides. The divalent alkali halides and remaining by-products, which account for a combined proportion of about 0.5 wt. %, are removed in a second step by means of ion exchange processes. The carrying out of the process according to the invention is described in the following in general form.

[0024] The Nanofiltration

[0025] The osmotic pressure of the reaction solution, which must be overcome during the nanofiltration for the depletion of the monovalent alkali halides, is determined by the concentration of the product, of the by-products and of the divalent alkali salts. In the usual reaction solution, this osmotic pressure amounts to about 30 bar. For the carrying out of the nanofiltration at technically and economically meaningful operating pressures, the reaction solution must, therefore, be diluted with water in a ratio of 1:2 to 1:3, whereby the osmotic pressure is reduced in the same way to 15-10 bar. The nanofiltration process is preferably carried out in the process manner of the diafiltration. For a sufficient depletion of the alkali halides, demineralised water is used as diafiltration solution. The diafiltrate volume corresponds to about 7 to 10 fold or the reaction solution. A depletion of the monovalent alkali halides to >95% is therewith possible. There is used a nanofiltration membrane with a cut-off of about 150-300 g/mol, preferably about 200 g/mol and an operating pressure of 25-35 bar, i.e. 15-20 bar higher than the osmotic pressure. Subsequently to the diafiltration, partly purified reaction solution is now concentrated to the original volume and further purified by ion exchange processes.

[0026] Ion Exchange Process

[0027] The exchange of anions and cations by means of ion exchange resins has long since been used and is prior art. The here-described purification is carried out with a combination of strongly acidic cation exchange and weakly basic anion exchange. There was used, for example, a strongly acidic ion exchanger of a type Pyrolite C 104 and, as weakly basic ion exchanger a type Pyrolite A 100. The manner of operation of the ion exchanger is to be carried out in a co-current or countercurrent process, the co-current principle is preferred. The ratio of anionic and cationic exchanger is so adjusted that the following anionic exchanger has the same exchanger capacity as the cationic exchanger. The capacity of the ion exchanger suffices in order to deplete a reaction volume pre-purified by the nanofiltration corresponding to the 8-10 fold bed volume of the cation exchanger. The ion exchange process makes possible a depletion of the alkali halides and by-products to less than 0.1%. For the elution of the product remaining in the ion exchanger, the ion exchanger columns are washed out with demineralised water. The wash water volume corresponds to about 2-3 fold bed volume, of the cation exchanger. The regeneration of the ion exchanger takes place according to the technical instructions of the manufacturer.

[0028] In the accompanying FIG. 1 is given the flow diagram of the combination according to the invention of nanofiltration and ion exchange for the product purification. In this Figure, the diafiltrate is passed over from the container 1 into the product container 2 from which it runs into the nanofiltration 3 in which the nanofiltration membrane 3 a is indicated by the diagonal line. The proportions not passing the membrane are returned into the product solution and there diluted back with diafiltrate to the purification concentration. The purified solution running through the membrane 3 a passes via an intermediate tank 4 to the first ion exchanger column with a strongly cationic exchanger 5 and its run-off to a second ion exchanger column with weakly anionic exchanger 6. The product purified by these ion exchangers is collected in the collection tank 7 and removed as required.

PROCESS EXAMPLE

[0029] Preparation of 3-morpholinopropane-sulphonic Acid Solution ω-(MOPS)

[0030] 6298 g (40 mol) of 1,3-bromo-chloropropane (BCP) was taken and warmed to 20° C., 420 g (10 mol) NaOH in 2000 ml water was added thereto and subsequently 870 g (10 mol) morpholine added thereto.

[0031] After 10 min, the suspension was heated to 45° C. It as stirred at this temperature for 8 hours.

[0032] 100 ml 33% hydrochloric acid in 1250 g water was added thereto in slight excess (about 11 mol). The amount of hydrochloric acid is thereby not to be observed but rather the pH value. First when this has sunk to 0 to 1, a sharp separation between aqueous and organic phase took place.

[0033] The organic phase (excess BCP) was run off.

[0034] To the aqueous phase is added concentrated soda lye up to a pH value of 5-6 and subsequently a saturated sodium sulphite solution (1260 g (10 mol) NaSO₃+1250 g H₂O) added thereto.

[0035] The mixture was heated to 75° C. and stirred at this temperature for 24 hours.

[0036] Thereafter, the solution was cooled and purified as follows.

[0037] Purification by Means of Nanofiltration and Ion Exchange

[0038] There were used 7.8 kg (6.5 litre) of the salt-containing product solution from the preparation of ω-MOPS. The product solution contained 1.21 kg ω-OPS, 0.73 kg NaCl, 0.6 kg NaBr and 0.04 kg Na₂SO₄. The separation of the monovalent salts NaCl and NaBr was carried out by means of nanofiltration. For the reduction of the osmotic pressure of the solution, the product solution was diluted with 6.5 litres demineralised water. The nanofiltration was carried out in the form of a diafiltration with a conventional spool module. There was used a spool module of the firm Osmonic with a cut-off of 250 Dalton and a membrane surface of 1 m². The nanofiltration took place in the case of a transmembrane pressure of 35 bar and a flowing over of the membrane of 1000 l/m²h.

[0039] The nanofiltration was carried out as follows: The salt-containing product solution was placed in the storage container of the nanofiltration plant and diluted with 6.5 litres of demineralised water. The permeate volume running off (average permeate flow 50 l/h) was continuously replaced in the storage container by demineralised water in the same ratio. (diafiltration). The nanofiltration was carried out until a permeate volume of 100 litres was removed from the system. The permeate was collected in a tank. Thereafter, a concentration of the now substantially desalinated product solution was carried out until the original volume of 6.5 litres was again reached. After the diafiltration of the reaction medium, a concentration of 100 litres of permeate to an end volume of 7.5 litres was carried out. The adjustment was here a membrane flowing over of 1000 l/m²h and a transmembrane pressure of 30 bar, The concentrated permiate and the partly desalinated product solution were thereafter mixed with one another (in the following called prepurified product solution), The permeate concentration had the purpose again to recover about 90% of the MOPS from the permeate and thus to increase the product-referred total recovery in the nanofiltration from 90% to over 99%.

[0040] After the nanofiltration, the mixture of partly desalinated product solution and concentrated permeate contained 1.20 kg MOPS, 0.045 kg NaCl, 0.037 kg NaBr and 0.04 kg Na₂SO₄. Thus, the nanofiltration makes possible a reduction of NaCl by 94%L and of NaBr of 95% in the case of a product loss smaller than 1%, the content of sodium sulphate is not reduced.

[0041] Subsequent to the nanofiltration, there took place the separating off of the sodium sulphate and of the remaining salts from the pre-purified solution by means of ion exchange processes. One litre of pre-purified product solution contained 0.144 kg ω-HOPS, 2.8 g NaCl, 3.5 g NaBr and 3 g Na₂SO₄. There was here used an arrangement of two columns with 350 ml of strongly cationic ion exchanger Relite EXCO8 and 170 ml of the weakly anionic exchanger Relite EXA54. The loading capacity of the ion exchange material suffices in order completely to desalinate 1 litre of pre-purified product solution. The ion exchangers are used in their correspondingly regenerated form (H-form for strongly cationic and OH-form for weakly anionic). The pre-purified product solution was first passed over the strongly cationic ion exchanger and thereafter over the weakly anionic ion exchanger. The flow throught of the pre-purified product solution through the ion exchangers took place by the hydrostatic pressure. Thereafter, both ion exchangers were rinsed with 1 litre of demineralised water. The rinse water and the now completely purified product solution are mixed with one another and this mixture gave 2 litres of product solution. After the purification by means of ion exchange processes, the product solution contained 0.138 kg ω-MOPS and no more detectable salts. This corresponded to a product loss smaller than 4%. The Purification was herewith ended. 

1. Process for the preparation of ω-aminoalkyl-sulphonic acids of the general formula I

in which R₁ and R₂ are the same or different and represent straight-chained or branched alkyl group with 1-20 C-atoms or, together with the nitrogen atom, form a 5- or 6-membered saturated aliphatic ring, 1 or 2 members of the ring can thereby also be N—R₃, O or S instead of CH₂, R₃ represents hydrogen or an alkyl group with 1-20 C-atoms and n represents a whole number from 2-6, characterised in that one reacts an amine of the formula II

in which R₁ and R₂ have the above meaning, with an alkyl dihalide of the formula III X₁—(CH₂)_(n)—X_(s)  III in which n has the above meaning, X₁ and X₂=chlorine or bromine, and alkali sulphite in aqueous solution, whereby first the amine and alkyl dihalide are brought to reaction with addition of alkali hyroxide at a pH value of 8-10 and subsequently, by addition of hydrohalic acid, a pH value of 0-1 is adjusted and excess alkyl dihalide is separated off before the reaction solution is adjusted with alkali lye to a pH value of 6-7.5, alkali sulphite is added thereto and the product of formula I is formed at elevated temperature.
 2. Process according to claim 1, characterised in that as alkali sodium is used and as halogen chloride.
 3. Process according to claim 1, characterised in that the reaction solution is separated by nanofiltration from alkali halides and sulphides contained.
 4. Process according to claim 1 or 2, characterised in that the reaction solution adjusted for the nanofiltration to a concentration with an osmotic pressure of 10-20 bar.
 5. Process according to claim 2 or 3, characterised in that after the nanofiltration there follows a purification by means of ion exchange chromatography, whereby, by means of a strongly acidic cation exchanger and a weakly basic anion exchanger, the bisulphides and nonamphoteric starting materials and by-products are separated off.
 6. Use of purified solutions according to claim 2-4 as biobuffers. 